Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana

Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana

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Y73 is a progeny of asymmetric powell and mahoney bloody mary mix somatic hybridization between Oryza sativa cv.Dalixiang and the wild rice species Oryza meyeriana.Inoculation with a range of strains of Xanthomonas oryzae pv.oryzae showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent.

An F2 population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24.After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism the gel bottle audrey between Y73 and IR24.Molecular markers linked to the BB resistance genes in Y73 were scanned using the F2 population and the polymorphic markers.The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.